Urinary microvesicles and exosomes have recently emerged as a potential source of biomarkers in renal health and disease. Still, the biological functions and signaling events facilitated through microvesicles and exosomes shed into the interstitium and primary urine, remain elusive. Therefore, we are investigating cell-specific subpopulations of renal microvesicles and exosomes for their biomolecular functions in vitro and in vivo. Likewise, we are establishing protocols for the isolation of the aforementioned subpopulations from urine samples in search for microvesicular proteins or nucleic acids that serve as novel biomarkers to accompany kidney biopsies in various nephropathies.  


Researchers:

Fabian Braun

Oliver Kretz

Harry Holthöfer






Related publications:

Purification and Identification of Membrane Proteins from Urinary Extracellular Vesicles using Triton X-114 Phase Partitioning.Hu S, Musante L, Tataruch D, Xu X, Kretz O, Henry M, Meleady P, Luo H, Zou H, Jiang Y, Holthofer H.J Proteome Res. 2018 Jan 5;17(1):86-96.

Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis.Mellows B, Mitchell R, Antonioli M, Kretz O, Chambers D, Zeuner MT, Denecke B, Musante L, Ramachandra DL, Debacq-Chainiaux F, Holthofer H, Joch B, Ray S, Widera D, David AL, Huber TB, Dengjel J, De Coppi P, Patel K. Stem Cells Dev. 2017 Sep 15;26(18):1316-1333.

Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries.Barreiro K, Holthofer H.Cell Tissue Res. 2017 Jul;369(1):217-227