The glomerulus represents a prime target of age-related tissue damage. Our preliminary results indicate that aging is associated with specific alterations of chromatin modifying enzymes in glomerular epithelial cells. The general role of epigenetic regulation will be elucidated by tissue specific ablation of DNA modifying enzymes. Correlative analysis of DNA methylation and gene expression from aged epithelial cells will help to generate a functional map of DNA methylation. Finally, the drosophila kidney like cell, the nephrocyte, will be used to efficiently screen for the function of genes being altered by DNA modifications with age.